| 1r | Miserere nostri Domine | Ferrabosco, Alfonso (the elder) (1543–1588) |
| 1v–2v | Miserere nostri Domine | Daman, William |
| 2v–4r | Fuggito e'l sonno | Marenzio, Luca (ca. 1553–1599) |
| 3v–5r | Senza il mio vago - 1a pars (of 2) | Marenzio, Luca (ca. 1553–1599) |
| 4v–6r | Senza il mio vago - 2a pars (of 2): O giorno | Marenzio, Luca (ca. 1553–1599) |
| 5v–7r | Fiere silvestre - 1a pars (of 2): Fiere silvestre | Marenzio, Luca (ca. 1553–1599) |
| 7r–8v | Fiere silvestre - 2a pars (of 2): Ecco che un'altra volta | Marenzio, Luca (ca. 1553–1599) |
| 8v–10r | E se di vero Amor | Marenzio, Luca (ca. 1553–1599) |
| 9v–11r | Co m'ogni rio che d'acque | Marenzio, Luca (ca. 1553–1599) |
| 11v–12v | Valli riposte | Marenzio, Luca (ca. 1553–1599) |
| 13r–16v | Basti fin qui le pen' e i duri affanni (10vv) | Marenzio, Luca (ca. 1553–1599) |
| 17r–18r | O fere stelle | Marenzio, Luca (ca. 1553–1599) |
| 18r–19r | Se la mia vita [1a pars only] | Marenzio, Luca (ca. 1553–1599) |
| 18v–20r | Piango ch'Amor | Marenzio, Luca (ca. 1553–1599) |
| 19v–20r | Affliger chi per voi (1a pars only) | Marenzio, Luca (ca. 1553–1599) |
| 20v–21r | O voi che sospirati | Marenzio, Luca (ca. 1553–1599) |
| 21r–22r | Dolorosi martir | Marenzio, Luca (ca. 1553–1599) |
| 22v–25v | Super flumina Babylonis | Monte, Philippe de |
| 22v–24r | In nomine | Taverner, John |
| 24r–25v | In nomine | Golder, Robert |
| 26r–30r | Quomodo cantabimus (2p. Si non proposuero Jersualem) | Byrd, William (ca. 1540–1623) |
| 30v–32r | Peccantem me quotidie | Byrd, William (ca. 1540–1623) |
| 31v–33r | Aspice Domine quia facta est (6vv) | Byrd, William (ca. 1540–1623) |
| 33r–36r | Attollite portas [= Let us arise from sin; or Lift up your heads; Psalm for Ascension Day evensong] | Byrd, William (ca. 1540–1623) |
| 35v–37r | Vias tuas (C25) | Ferrabosco, Alfonso (the elder) (1543–1588) |
| 36v–39r | Vestigia mea dirige | Giles, Nathaniel (ca. 1558–1634) |
| 38v–40r | Tibi soli | Giles, Nathaniel (ca. 1558–1634) |
| 39v–40r | My soul oppressed | Byrd, William (ca. 1540–1623) |
| 40v–45r | Domine quis habitabit | Byrd, William (ca. 1540–1623) |
| 45v–46v | Amavit eum Dominus | Tye, Christopher (ca. 1505–ca. 1573) (?) |
| 46v–49r | Memento Domine | Byrd, William (ca. 1540–1623) |
| 48v–51r | Tristitia et anxietas - 1a pars (of 2): Tristitia et anxietas, Domine tu jurasti | Byrd, William (ca. 1540–1623) |
| 51r–52v | Tristitia et anxietas - 2a pars (of 2): Sed tu domine | Byrd, William (ca. 1540–1623) |
| 52v–53v | Ultimi miei sospiri che mi lasciate | Ferrabosco, Alfonso (the elder) (1543–1588) (?) / [or] Verdelot, Philippe (ca. 1480–ca. 1532) |
| 53v–54r | Salva me - 1a pars (of 2): Salva me | Ferrabosco, Alfonso (the elder) (1543–1588) |
| 54r–54v | Salva me - 2a pars (of 2):Christe redemptor | Ferrabosco, Alfonso (the elder) (1543–1588) |
| 54v–56r | Deus misereatur nostri - 1a pars (of 2): Deus misereatur nostri et benedicat nobis | White, Robert (ca. 1538–1574) |
| 55v–57v | Deus misereatur nostri - 2a pars (of 2): Laetentur et exsultent | White, Robert (ca. 1538–1574) |
| 57v–58v | Adolescentulus sum ego | Mundy, William (ca. 1528–ca. 1591) |
| 58v–59r | Tribulacio | Mundy, William (ca. 1528–ca. 1591) |
| 59r–59v | Iudica me Deus | Mundy, John (ca. 1555–1630) |
| 59v–60r | Haec dies | Sheppard, John (ca. 1515–1558) |
| 60v–61r | Missa 'Gloria tibi Trinitas': Credo | Taverner, John |
| 60v–61r | Missa 'Gloria tibi Trinitas': Sanctus | Taverner, John |
| 61v–62v | Loquebantur variis linguis | Tallis, Thomas (ca. 1505–1585) |
| 63r–64v | In nomine | Mundy, John (ca. 1555–1630) |
| 63v–65r | In nomine | Mundy, John (ca. 1555–1630) |
| 64v–65v | In nomine | Mundy, John (ca. 1555–1630) |
| 65v–66v | In nomine | Mundy, John (ca. 1555–1630) |
| 66v–67v | Gaude Maria virgo - 1a pars (of 2): Gaude Maria virgo | Morley, Thomas (ca. 1557–1602) |
| 67v–69r | Gaude Maria virgo - 2a pars (of 2): Virgo prudentissima | Morley, Thomas (ca. 1557–1602) |
| 68v–70r | Ne irascaris - 1a pars (of 2): Ne irascaris Domine | Byrd, William (ca. 1540–1623) |
| 69v–71r | Ne irascaris - 2a pars (of 2): Civitatis sancti tui | Byrd, William (ca. 1540–1623) |
| 70v–71v | Tribulationes civitatum - 1a pars (of 3): Tribulationes civitatum | Byrd, William (ca. 1540–1623) |
| 71v–72v | Tribulationes civitatum - 2a pars (of 3): Timor et hebitudo | Byrd, William (ca. 1540–1623) |
| 72v–73r | Tribulationes civitatum - 3a pars (of 3): Nos enim pro peccatis | Byrd, William (ca. 1540–1623) |
| 73v–74r | Omni tempore - 1a pars (of 2): Omni tempore benedic Deum | Byrd, William (ca. 1540–1623) |
| 74r–75r | Omni tempore - 2a pars (of 2): Memor esto fili | Byrd, William (ca. 1540–1623) |
| 74v–75v | Ne perdas - 1a pars (of 2): Ne perdas cum impiis | Byrd, William (ca. 1540–1623) |
| 75v–76r | Ne perdas - 2a pars (of 2): Eripe me | Byrd, William (ca. 1540–1623) |
| 76r | [Untitled] | Taverner, John |
| 76v–77v | Libera me - 1a pars (of 2): Libera me Domine de morte aeterna | Parsons (I), Robert |
| 77v–78r | Libera me - 2a pars (of 2): Dies illa | Parsons (I), Robert |
| 78r–79r | Aspice Domine (5vv) - 1a pars (of 2): Aspice Domine de sede sancta | Byrd, William (ca. 1540–1623) |
| 78v–79r | Aspice Domine (5vv) - 2a pars (of 2): Respice domine | Byrd, William (ca. 1540–1623) |
| 79v–80r | O quam gloriosum - 1a pars (of 2): O quam gloriosum est regnum | Byrd, William (ca. 1540–1623) |
| 80r–81r | O quam gloriosum - 2a pars (of 2): Benedictio et claritas [same link as previous] | Byrd, William (ca. 1540–1623) |
| 80v–82v | Deus omnipotens | Bull, John (ca. 1562–1628) |
| 82v–83r | I am feeble | Bull, John (ca. 1562–1628) |
| 82v–83v | Great Service: Te Deum part 1 | Byrd, William (ca. 1540–1623) |
| 83v | Great Service: Te Deum part 2 | Byrd, William (ca. 1540–1623) |
| 83v–84r | Great Service: Te Deum part 3 | Byrd, William (ca. 1540–1623) |
| 84r–85r | Great Service: Te Deum part 4 | Byrd, William (ca. 1540–1623) |
| 85r–85v | Missa Cantate Domino: Benedictus | Sheppard, John (ca. 1515–1558) |
| 84v–85v | In nomine | Baldwin, John (ca. 1560–1615) |
| 86r | Great Service: And ye Child | Byrd, William (ca. 1540–1623) |
| 86r | Great Service: That we should be | Byrd, William (ca. 1540–1623) |
| 86v–87r | [Untitled] | Anonymous |
| 87v–89r | [Untitled] | Anonymous |
| 89v–90r | If Reason did Rule | Anonymous |
| 90v–91r | The Batchelar Most Joyfully | Anonymous |
| 90v–91r | If he be merry and toy with any | Anonymous |
| 91v–92r | Dicant nunc Judei | Byrchley |
| 91v–92r | Missa Te Deum: Agnus dei | Aston, Hugh |
| 92v–93r | Dicant nunc Judei | Johnson (I), Robert (ca. 1500–ca. 1560) |
| 92v–93r | Dicant nunc Judei | Gore |
| 93v–94r | Ego sum panis | Anonymous |
| 93v–94r | Sancti spiritus Domine | Anonymous |
| 93v–94r | Jesu salvator mundi | Anonymous |
| 94v–97r | Laudes Deo | Johnson (I), Robert (ca. 1500–ca. 1560) |
| 96v–97r | Dicant nunc Judei | Johnson (I), Robert (ca. 1500–ca. 1560) |
| 96v–97r | Agnus Dei | Anonymous |
| 97v–98r | Agnus Dei | Barber |
| 97v–98r | Agnus Dei | Anonymous |
| 97v–98r | Agnus Dei | Anonymous |
| 98v–99r | A duo | Baldwin, John (ca. 1560–1615) |
| 99v–100r | In Women is Rest | Taverner, John |
| 100v–101r | A duo | Baldwin, John (ca. 1560–1615) |
| 101v–102r | Ut re mi fa | Woodson, Thomas |
| 101v–102r | A duo | Baldwin, John (ca. 1560–1615) |
| 102v–103r | Miserere | Giles, Nathaniel (ca. 1558–1634) |
| 103v | Exaudi me | Anonymous |
| 104r | In nomine 'Hold fast' | Tye, Christopher (ca. 1505–ca. 1573) |
| 104v–105r | Vide domine | Bedingham, John (–ca. 1460) |
| 105r | Parce domine | Anonymous |
| 105v–106r | Manus Dei | Bedingham, John (–ca. 1460) |
| 106v–107r | Salve Jesu | Bedingham, John (–ca. 1460) |
| 106v | A duo | Anonymous |
| 107v–108r | In te domine speravi | Giles, Nathaniel (ca. 1558–1634) |
| 108v–109r | Spes mea | Baldwin, John (ca. 1560–1615) |
| 109v–110r | In manus tuas domine | Baldwin, John (ca. 1560–1615) |
| 110v | Salva nos | Anonymous |
| 111r | Salva nos | Anonymous |
| 111v–112r | Salvator mundi | Giles, Nathaniel (ca. 1558–1634) |
| 112v–113r | Sermone blando : angelus | Baldwin, John (ca. 1560–1615) |
| 113v–115r | Sit fast | Tye, Christopher (ca. 1505–ca. 1573) |
| 115v–118r | [3] Kyries of 3 voices | Anonymous |
| 118v–119r | O lux beata Trinitas | Preston, Thomas (–ca. 1559) |
| 118v–119r | Ut re mi fa sol la (2p. La sol fa mi re ut) | Ferrabosco, Alfonso (the younger) (ca. 1575–1628) |
| 119v–120r | A Fancy | Baldwin, John (ca. 1560–1615) |
| 120v–121r | Browning a3 | Bevin, Elway |
| 121v–122r | A Browning of 3 voc | Baldwin, John (ca. 1560–1615) |
| 122v–123r | O lux | Baldwin, John (ca. 1560–1615) |
| 123v–124r | Upon in nomine | Baldwin, John (ca. 1560–1615) |
| 124v–125r | Cuckoo as I me walked | Baldwin, John (ca. 1560–1615) |
| 125v–126r | 3 voices upon the Plainsong | Baldwin, John (ca. 1560–1615) |
| 126v–127r | [Untitled] | Bull, John (ca. 1562–1628) |
| 127v–128r | Proportions to the Minim | Baldwin, John (ca. 1560–1615) |
| 128v–134r | Mass for 3 voices | Byrd, William (ca. 1540–1623) |
| 134v–135r | Gaude plurimum [2p. Gaude Maria virgo, 3p. Gaude Maria, Jesu mater] | Taverner, John |
| 135r | Eundem igitur Jesum | Taverner, John |
| 135v–136r | Illustrissima omnium (fr. 1 from a lost antiphon) | Sheppard, John (ca. 1515–1558) |
| 136r | Missa O bone Jesu: Genitum non factum (from Credo) | Fayrfax, Robert |
| 136v–137r | Dominus illuminacio mea | Mundy, John (ca. 1555–1630) |
| 137v–138r | Exsurge Domine (extract: opening duo) | Wood, John |
| 138r–139v | Noli aemulari in malignantibus | Mundy, William (ca. 1528–ca. 1591) |
| 139v–140r | Vox Patris caelestis | Mundy, William (ca. 1528–ca. 1591) |
| 140v–141r | Maria virgo sanctissima | Mundy, William (ca. 1528–ca. 1591) |
| 140v–141r | Quis est homo | Giles, Nathaniel (ca. 1558–1634) |
| 141v–142r | Exsurge Domine (extract: Esto pater / Querum parturbate ) | Wood, John |
| 141v–142r | In manus tuas | Parsons (I), Robert |
| 142v–143r | Exsurge Domine (extract: v3, Perfice illud quod in nobis) | Wood, John |
| 142v–143r | Traditur militibus From lost sequence | Taverner, John |
| 143v–144r | Maria virgo sanctissima | Mundy, William (ca. 1528–ca. 1591) |
| 143v–144r | Adhesit pavimento | Mundy, William (ca. 1528–ca. 1591) |
| 144v–145r | Vox Patris caelestis | Mundy, William (ca. 1528–ca. 1591) |
| 144v–145r | Tam peccatum | Taverner, John |
| 145v–146r | Exsurge Domine (extract: v4, Verbi tui fulminibus) | Wood, John |
| 145v–146r | Virgo pura | Taverner, John |
| 146v–147r | Magnus es domine | Parsons (I), Robert |
| 146v–147r | Maria plena virtute: (extract) Rex amabilis | Fayrfax, Robert |
| 147v–148r | Et cum pronobis | Tye, Christopher (ca. 1505–ca. 1573) |
| 147v–148r | Jesu spes penitentibus | Taverner, John |
| 148v–149r | O splendor gloriae | Tye, Christopher (ca. 1505–ca. 1573) (?) / [or] Taverner, John (?) |
| 148v–149r | Gloriosa domine | Taverner, John |
| 149v–150r | Prudens virgo taces | Taverner, John |
| 149v–150r | Tu ad liberandum | Taverner, John |
| 150v–151r | Te angelorum domina | Taverner, John |
| 150v–151r | Ergo Sathan mors peccatum | Mundy, John (ca. 1555–1630) |
| 151v–152r | Domine Deus caelestis - 1a pars (of 2): Domine Deus caelestis | Tye, Christopher (ca. 1505–ca. 1573) |
| 151v–152r | Domine Deus caelestis - 2a pars (of 2): Da illi domine pectus tuo | Tye, Christopher (ca. 1505–ca. 1573) |
| 152v–153r | In quo corriget | Tye, Christopher (ca. 1505–ca. 1573) |
| 152v–153r | In corde meo abscondi | Anonymous |
| 152v–153r | Manus tuae fecerunt me [2p. Veniant mihi] | White, Robert (ca. 1538–1574) |
| 153v–154r | Quales sumus: (extract) Ut te laudare presumamus | Mason, John |
| 153v–154r | Quales sumus: (extract) Quare pro nobis | Mason, John |
| 154v–155r | Maria plena virtute - 1a pars (of 2): Maria plena virtute | Anonymous |
| 154v–155 | Maria plena virtute - 2a pars (of 2): Rex amabilis | Anonymous |
| 155v–156r | Gaude gloriosa Dei mater (extract: opening trio, Gaude gloriosa Dei mater) | Tallis, Thomas (ca. 1505–1585) |
| 155v–156r | Ave Dei patris filia (extract, trio: Ave summae aeternitatis filia) | Fayrfax, Robert |
| 156v–157r | Gaude gloriosa Dei mater (extract: trio, Gaude Virgo Maria quae corpore et anima) | Tallis, Thomas (ca. 1505–1585) |
| 156v–157r | Ave Dei patris filia (extract, opening trio) | Fayrfax, Robert |
| 157v–158r | Gaude plurimum - 2a pars (of 3): Gaude Maria virgo | Taverner, John |
| 158v–159r | Inclina Domine aurem tuam (I) | Sheppard, John (ca. 1515–1558) |
| 158v–159r | Salve intemerata virgo - 4a pars (of 4): Secundum humanitatem | Tallis, Thomas (ca. 1505–1585) |
| 159v–160r | Salve intemerata virgo - 2a pars (of 4): Tu nimirum | Tallis, Thomas (ca. 1505–1585) |
| 159v–160r | Salve intemerata virgo - 3a pars (of 4): Per haec nos | Tallis, Thomas (ca. 1505–1585) |
| 160v–161r | Alleluia. Confitemini Domino quoniam bonus | Byrd, William (ca. 1540–1623) |
| 160v–161r | Cunctis diebus [2p. Dimitte me ergo] | Byrd, William (ca. 1540–1623) |
| 160v–161r | Infelix ego omnium - 1a pars (of 3): Infelix ego omnium | Byrd, William (ca. 1540–1623) |
| 161v–163r | Stella caeli | Thorne, John |
| 162v–163r | Stella caeli [2p. Ipsa stella; 3p. O gloriosa; 4p. Nam te filius 5p. Salva nos Jesu] | Cowper (Cooper), Robert (ca. 1474–ca. 1540) |
| 163v–164r | O crux gloriosa | Cowper (Cooper), Robert (ca. 1474–ca. 1540) |
| 164v–165r | Gloria laus | Moorecocke |
| 165v–166r | Ad lapidis posicionem | Dygon, John |
| 165v–166r | Rex benedicte tuos | Dygon, John |
| 166v–167r | Quam pulchra es | Henry VIII (1491–1547) |
| 167v–169r | If Reason Did Rule | Baldwin, John (ca. 1560–1615) |
| 169v–171r | I Had Both Money and a Friend | Bevin, Elway |
| 171v–173r | In the Merry Month of May | Baldwin, John (ca. 1560–1615) |
| 173v–175r | Lord Who Shall Dwell | Baldwin, John (ca. 1560–1615) |
| 175v–177r | Out of the Deep [2p. My soul flyeth to the lord] | Giles, Nathaniel (ca. 1558–1634) |
| 177v–178r | Save me O God | Baldwin, John (ca. 1560–1615) |
| 178v–180r | Behold How Good | Baldwin, John (ca. 1560–1615) |
| 180v–181r | Behold How Good | Baldwin, John (ca. 1560–1615) |
| 181v–183r | Lord Who Shall Dwell | Bevin, Elway |
| 183v–184r | By Mirth Much Sickness | Bevin, Elway |
| 184v–186r | What Comfort at thy Death II: Death thus to suffer | Sheppard, John (ca. 1515–1558) |
| 186v–187r | A Virgin and Mother | Merbecke, John |
| 187v–188r | Steven First After Christ | Sheppard, John (ca. 1515–1558) |
| 188v | Jesus autem transiens | Wylkynson, Robert |